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Internalization of PLGA nanoparticles coated with poloxamer 188 in glioma cells: A confocal laser scanning microscopy study
Affiliation:1. D. I. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russian Federation;2. V. P. Serbsky National Medical Research Centre of Psychiatry and Narcology, 119034 Moscow, Russian Federation;3. N. I. Pirogov Russian National Research Medical University, 117997 Moscow, Russian Federation;1. Department of Endodontics, Faculty of Dentistry, Marmara University, Istanbul, Turkey;2. Endodontology Research Group, School of Dentistry, Cardiff University, Cardiff, UK;3. Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran;1. Department of Endodontics, Faculty of Dentistry, Marmara University, Istanbul, Turkey;2. Endodontology Research Group, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff, United Kingdom;3. Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran;1. A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, 119334 Moscow, Russian Federation;2. D. I. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russian Federation;3. M. M. Shemyakin–Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russian Federation;4. Department of Chemistry, M. V. Lomonosov Moscow State University, 119991 Moscow, Russian Federation;1. Institute of Macromolecular Compounds, Russian Academy of Sciences, 199004 St. Petersburg, Russian Federation;2. Saint-Petersburg State University of Industrial Technologies and Design, 191186 St. Petersburg, Russian Federation;3. St. Petersburg State Institute of Technology (Technical University), 190013 St. Petersburg, Russian Federation;4. Institute of Earth Sciences, St. Petersburg State University, 199034 St. Petersburg, Russian Federation
Abstract:Internalization of poloxamer 188-coated PLGA nanoparticles (NPs) in GL261 murine glioma cells was studied using confocal laser scanning microscopy. For visualization, both poloxamer 188 (P188) and PLGA were labeled covalently with fluorescent dyes Rhodamine B and Cyanine5, respectively. The results indicated that the PLGA NPs coated with poloxamer 188 enter a cell as an integral core–shell structure, which can be helpful for gaining further insight into the in vivo performance of surfactant-coated polymeric NPs as core–shell delivery systems
Keywords:poloxamer 188  confocal laser scanning microscopy (CLSM)  polylactide-co-glycolide nanoparticles (PLGA NPs)  GL261 murine glioma cells  Rhodamine B
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