Determination of l-lactate by differential pulse polarography with covalently immobilized enzyme on Chitopearl |
| |
Authors: | Kiyoshi Hasebe Satoshi Hikima and Hitoshi Yoshida |
| |
Institution: | (1) Department of Chemistry, Faculty of Science, Hokkaido University, 060 Sapporo, Japan |
| |
Abstract: | Summary The covalent bonding of l-lactate oxidase (no E.C.) to cross-linked copolymers of chitosan (Chitopearl) using the glutaraldehyde method is described and applied to the determination of l-lactate in serums of diseased and normal animals. The bioreactor packing of the immobilized enzyme is stable for at least 4 months and can be continuously used for the rapid and simple determination of l-lactate. The reduction current of pyruvate, corresponding to the product of enzymatic reaction, is determined by differential pulse polarography. The reproducibility (RSD) for 10 mol/l l-lactate is 0.78% (n=6) and the detection limit is 0.62 mol/l (k=2, confidence level 97.72%). |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|