Extractive Fermentation of Xylanase from Aspergillus tamarii URM 4634 in a Bioreactor |
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Authors: | Anna Carolina da Silva Alana Emília Soares de França Queiroz Talita Camila Evaristo dos Santos Nascimento Cristine Rodrigues José Erick Galindo Gomes Cristina Maria Souza-Motta Luciana Porto de Souza Vandenberghe Erika Valente de Medeiros Keila Aparecida Moreira Polyanna Nunes Herculano |
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Institution: | 1. Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco, Av. Bom Pastor, s/n, Boa Vista, CEP 55.292-270, Garanhuns, PE, Brazil 2. Universidade Federal do Paraná/Centro Politécnico, Av. Coronel Francisco Heráclito dos Santos, 210-Jardim das Américas, CEP 81531-970, Curitiba, PR, Brazil 3. Departamento de Micologia, Universidade Federal de Pernambuco, Av. Moraes Rego, s/n, CEP 50670-901, Recife, PE, Brazil 4. Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irm?os, CEP 52171-900, Recife, PE, Brazil
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Abstract: | Of the many reported applications for xylanase, its use as a food supplement has played an important role for monogastric animals, because it can improve the utilisation of nutrients. The aim of this work was to produce xylanase by extractive fermentation in an aqueous two-phase system using Aspergillus tamarii URM 4634, increasing the scale of production in a bioreactor, partially characterising the xylanase and evaluating its influence on monogastric digestion in vitro. Through extractive fermentation in a bioreactor, xylanase was obtained with an activity of 331.4 U mL?1 and 72 % yield. The xylanase was stable under variable pH and temperature conditions, and it was optimally active at pH 3.6 and 90 °C. Xylanase activity potentiated the simulation of complete monogastric digestion by 6 %, and only Mg2+ inhibited its activity. This process provides a system for efficient xylanase production by A. tamarii URM 4634 that has great potential for industrial use. |
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