Optimizing methylation conditions for gas liquid chromatography assay of lactic and succinic acid in biological samples |
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| Authors: | K. S. Bricknell I. Brook S. M. Finegold |
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| Affiliation: | (1) Department of Medicine, UCLA School of Medicine, 90024 Los Angeles, California;(2) Medical Service, VA Wadsworth Hospital Center, 90073 Los Angeles, California, USA;(3) Present address: Clinical Laboratory, Children's Hospital National Medical Center, 20010 Washington, D.C., USA |
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| Abstract: | Summary Aqueous standard solutions of lactic and succinic acids were methylated for assay by gas-liquid chromatography. The efficiency of methylation using boron trifluoridemethanol reagent was compared to methylation using a sulfuric acid-methanol mixture; the ratio of sulfuric acid and methanol was also varied. Reactions were done at 100°C/5 min and at room temperature/20 hours. We found the sulfuric acid-methanol was most efficient for a rapid assay; however, the ratio of sulfuric acid and methanol used is critical in the assay of lactic acid and would be especially important in the clinical laboratory using gas-liquid chromatography for analysis of cerebrospinal fluid from suspect meningitis patients or other body fluids. |
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