Probing supported model and native membranes using AFM |
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Authors: | Bastien Seantier Marie-Ccile Giocondi Christian Le Grimellec Pierre-Emmanuel Milhiet |
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Institution: | aINSERM, Unité 554, Montpellier, France, Université de Montpellier, CNRS, UMR 5048, Centre de Biochimie Structurale, Montpellier, France |
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Abstract: | Atomic Force Microscopy (AFM) has gained lots of interest since its ability to get high resolution imaging in liquid environment. In the last years, this technique was particularly successful in probing the surface of membrane model systems of biological interest and spectacular results have been obtained with native specialized membranes. In this review, we aim at highlighting the recent developments that illustrate the unique powerfulness of AFM in determining the nanoscale organization of membranes and their local physical properties. An important part will focus on AFM high resolution imaging of transmembrane proteins in model and native membranes and on the study of few applications such as biosensors. An overview of main recent developments of AFM as well as new possibilities gained by combination with other techniques will also be addressed. |
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Keywords: | Atomic force microscopy Model membranes Native membranes Supported lipid bilayer Transmembrane proteins |
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