| 胡萝卜悬浮培养细胞和原生质体的玻璃化法超低温保存 |
| |
| 引用本文： | 陈勇,王君晖,黄纯农. 胡萝卜悬浮培养细胞和原生质体的玻璃化法超低温保存[J]. 浙江大学学报(理学版), 2002, 29(1): 94-98 |
| |
| 作者姓名： | 陈勇 王君晖 黄纯农 |
| |
| 作者单位： | 浙江大学,生命科学学院,浙江,杭州,310028 |
| |
| 基金项目： | 国家自然科学基金资助项目 (No.3990 0 0 12 ) |
| |
| 摘 要： | 采用玻璃化法成功地超低温保存了胡萝卡悬浮培养细胞及其原生质体，存活率分别为83．3％（TTC法检测）和47％（RDA法检测）。冻后细胞能恢复生长并再生植株。分析了玻璃化法超低温保存植物悬培养浮细胞和原生质体的主要影响因素。
|
| 关 键 词： | 玻璃化法 超低温保存 胡萝卜 悬浮培养细胞 原生质体 植株再生 低温生物学 |
| 文章编号： | 1008-9497(2002)01-0094-05 |
| 修稿时间： | 2000-03-16 |
Cryopreservation by vitritication of carrot (Daucus carota L.) suspension cells and protoplasts. |
| |
| CHEN Yong,WANG Jun|hui,HUANG Chun|nong. Cryopreservation by vitritication of carrot (Daucus carota L.) suspension cells and protoplasts.[J]. Journal of Zhejiang University(Sciences Edition), 2002, 29(1): 94-98 |
| |
| Authors: | CHEN Yong WANG Jun|hui HUANG Chun|nong |
| |
| Abstract: | The survival rates for carrot cells and protoplasts cryopreserved by vitrification were 83.3% (based on the TTC reduction) and 47% (based on the FDA assay) respectively. The frozen cells regrew and regenerated green plantlets. Factors related to the survival rates of vitrification of carrot cells and protoplasts are presented, including preculture, dehydration, loading and unloading. |
| |
| Keywords: | vitrification cryopreservation carrot cell suspension protoplast plant regeneration. |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《浙江大学学报(理学版)》浏览原始摘要信息 |
|
点击此处可从《浙江大学学报(理学版)》下载全文 |
