| bop基因及其上游启动子的克隆与表达 |
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| 引用本文： | 宋景娇,王友亮,曹军卫.bop基因及其上游启动子的克隆与表达[J].武汉大学学报(理学版),2005,51(2):215-218. |
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| 作者姓名： | 宋景娇 王友亮 曹军卫 |
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| 作者单位： | 武汉大学,生命科学学院,湖北,武汉,430072 |
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| 摘 要： | 应用重组PCR的方法，直接从盐沼盐杆菌(Halobactrium salinarium)S9的基因组DNA上扩增到了编码细菌视紫红质(bacteriorhodopsin)的基因——bop基因及其启动子部分(1196bp)，并将其克隆到表达载体pXLNovR后，在宿主菌株中得到了表达．测序结果表明，扩增得到的片段与NCBI数据库中的bop基因及启动子的序列完全相同，且其表达产物的分子量与野生型BR蛋白的分子量一致，并且在568nm处表现出BR蛋白特征吸收峰．本方法与其他方法(如逆转录PCR，建立基因文库后从中调取等方法)相比具有操作简便，成功率高的优点．
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| 关 键 词： | 细菌视紫红质 bop基因 重组PCR BR蛋白 |
| 文章编号： | 1671-8836(2005)02-0215-04 |
| 修稿时间： | 2004年12月13 |
Cloning and Expression of Bacterio-Opsion Gene |
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| SONG Jing-jiao,WANG You-liang,CAO Jun-Wei.Cloning and Expression of Bacterio-Opsion Gene[J].JOurnal of Wuhan University:Natural Science Edition,2005,51(2):215-218. |
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| Authors: | SONG Jing-jiao WANG You-liang CAO Jun-Wei |
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| Abstract: | In the present study, the bop gene and the promotor were amplified directly from the genomic DNA of Halobactrium salinarium S9 by recombinant-PCR method. Then, we constructed a recombinant plasmid pXLNovR-bop and expressed bop gene in the L33 cells. The length of the sequence is1196 base pair which is the same with the sequence of bop gene in the NCBI database. The molecular weight of the BR protein expressed from the cloned bop gene is also the same with the BR protein of the Halobactrium salinarium S9 which shows a characteristic absorption peak located at 568nm.This method is easier and reliable than other methods. |
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| Keywords: | bacteriorhodopsin bop gene recombinant-PCR BR protein |
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