Fast separation of amino acid phenylthiohydantion derivatives by HPLC on a non-porous stationary phase |
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Authors: | B Boros K Kovasc R Ohmacht |
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Institution: | (1) Institute of Medical Chemistry, University Medical School, 7624 Pecs, Hungary |
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Abstract: | Summary A new method is described for the separation of phenylthiohydantoin (PTH) derivatives of the 20 common amino acids. The analysis
requires approximately 7 minutes and good resolution is obtained by RP-HPLC on columns packed with a non-porous stationary
phase (Kovasil-C14; 33×4.6 mm). Gradient elution was chosen with eluents containing either sodium acetate/ acetic acid buffers
(moderately acidic conditions) or a heptafluorobutyric acid modifier (strongly acidic eluent). A slightly different elution
order of the PTH-amino acids was found in the two systems. Low detection limits (in the femtomol range) were achieved with
simple commercial HPLC equipment.
Presented at the Balaton Symposium on High Peformance Separtion Methods, Siófok, Hungary, september 1–3, 1999 |
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Keywords: | Column liquid chromatography Non-porous stationary phases Phenylthiohydantoin (PTH) amino acid separation |
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