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| 碳酸钙结晶对胃蛋白酶二级结构的影响 | |
| 引用本文： | 朱书法,汤俊明,马晓明,郭玉明,张秀英,杨林.碳酸钙结晶对胃蛋白酶二级结构的影响[J].光谱学与光谱分析,2003,23(3):477-480. |
| 作者姓名： | 朱书法 汤俊明 马晓明 郭玉明 张秀英 杨林 |
| 作者单位： | 1. 河南师范大学化学与环境科学学院,河南,新乡,453002 2. 河南省高等学校环境科学与工程重点学科开放实验室,河南,新乡,453002 3. 河南师范大学化学与环境科学学院,河南,新乡,453002;河南省高等学校环境科学与工程重点学科开放实验室,河南,新乡,453002 |
| 基金项目： | 国家自然科学基金资助项目 (2 9971 0 0 9) |
| 摘 要： | 本文通过傅里叶变换红外光谱及退卷积、曲线拟合等技术研究了碳酸钙结晶对胃蛋白酶二级结构的影响 ,结果表明 :在纯的胃蛋白酶中 ,α 螺旋、 β 折叠、转角及无规卷曲等四种结构的含量分别为2 4 38% ,2 9 91% ,39 2 2 % ,6 4 9% ;而在CaCO3 胃蛋白酶溶液中 ,四种结构的含量分别为 2 0 9% ,93 30 4 % ,4 6 0 %和 0 0 0 6 %。由此可以看出 :碳酸钙晶体的形成使胃蛋白酶的α 螺旋结构减少 ,β 折叠结构增多 ,本文讨论了这种变化的本质。 |
| 关 键 词： | 生物矿化 碳酸钙 胃蛋白酶 二级结构 |
| 文章编号： | 1000-0593(2003)03-0477-04 |
| 修稿时间： | 2002年4月22日 |
The Effect of Crystallization of Calcium Carbonate on the Secondary Structure of Pepsin |
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| ZHU Shu-fa,TANG Jun-ming,MA Xiao-ming,GUO Yu-ming,ZHANG Xiu-ying and YANG Lin , .College of Chemistry and Environmental Science,Henan Normal University,Xinxiang ,China .Key Laboratory of Environmental Science and Engineering Education Commission of Henan,Xinxiang ,China.The Effect of Crystallization of Calcium Carbonate on the Secondary Structure of Pepsin[J].Spectroscopy and Spectral Analysis,2003,23(3):477-480. | |
| Authors: | ZHU Shu-fa TANG Jun-ming MA Xiao-ming GUO Yu-ming ZHANG Xiu-ying and YANG Lin College of Chemistry and Environmental Science Henan Normal University Xinxiang China Key Laboratory of Environmental Science and Engineering Education Commission of Henan Xinxiang China |
| Institution: | College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453002, China. |
| Abstract: | The effect of crystallization of calcium carbonate on the secondary structure of pepsin was studied by Fourier transform infrared spectroscopy, derivative, deconvolution and curve-fitting techniques in this paper. The result shows that the pure protein is composed of 24.38% alpha-helices, 29.91% beta-sheets, 39.22% beta-turns and 6.49% random structures, and the pepsin in the CaCO3/pepsin solution is composed of 2.09% alpha-helices, 93.304% beta-sheets, 4.60% beta-turns and 0.006% random structures. From these data we can see that the alpha-helices decrease and the beta-turns increase with the formation of the crystal of calcium carbonate. The essence of these changes is discussed in the paper. |
| Keywords: | Biomineralization Calcium carbonate Pepsin Secondary structure |
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