Preparation of '2,('3)-O-Acyl-pCpA derivatives as substrates for T4 RNA ligase-mediated “chemical aminoacylation” |
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| Authors: | TG Heckler LH Chang Y Zama T Naka SM Hecht |
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| Institution: | Departments of Chemistry and Biology, University of Virginia, Charlottesville, VA 22901, U.S.A. |
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| Abstract: | The synthesis of '2('3)-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of E. coli tRNA phe-COH. The modified (N-acetylammo)acyl-tRNA's so derived are characterized by their chromatographic and biochemical properties, and by their ability to act as P-site tRNA donors in the peptidyltransferase reaction. |
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