Preparation and storage of125I labelled endothelin |
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Authors: | Gao Feng Zhou Meiying Zhao Xialing Zhang Sunxi Wang Guoping |
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Institution: | (1) Academia Sinica, Shanghai Institute of Nuclear Research, Shanghai, China;(2) P.O. Box 800-204, 201800 Shanghai, (P.R. China);(3) Beijing Medical University, Beijing, (P.R. China) |
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Abstract: | Endothelin (ET) is a novel endothelium-derived vasoconstrictive peptide, purified from porcine aortic endothelial cells.125I-ET was prepared by lodogen method and purified by HPLC, the specific activity was 62.9 TBq/mmol. The stability of125I-ET was investigated in different storage conditions by radioimmunoassay. |
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