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A novel method for immediate post-purification purity determination of fractions collected during high-throughput purification |
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| Authors: | Yuan Ding Petersen Chris Rynd Matthew Mainquist James Meyer Andrew Isbell John J |
| Affiliation: | Department of Analytical and Bioanalytical Chemistry, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA. |
| Abstract: | Following purification, the fractions of purified samples typically are analyzed to determine the relative purities of each fraction. We report a novel technique for performing post-purification analysis immediately after each preparative LC/MS run. The Single Pass Compound Purification and Analysis System (SPACPASS) samples and stores a representative aliquot from the fraction while it is being collected. Demonstrated for '1:1' fraction collections, this method of fraction purity assessment streamlined sample processing by reducing post-purification sample handling. For 97% of the collected fractions, this technique provided relative purities to within +/-5% when compared with more traditional post-purification analysis. |
| Keywords: | High throughput purification and analysis High throughput purification Fraction analysis Single pass purification and analysis HPLC/MS purification LC/MS purification Mass-directed fraction collection Prep LC/MS SPACPASS Representative sampling Representative flow stream sampling Purity |
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