明胶在聚氨酯表面的固定化及其对内皮细胞生长的促进作用

理想的组织工程支架材料应具备有效促进细胞生长的能力和良好的组织相容性 .然而现有的聚合物生物材料大多呈现疏水性 ,不能有效支持细胞的生长 1,2 ] .细胞外基质和血清中含有对细胞粘附、生长和繁殖有显著促进作用的多种活性因子 ,如纤维粘连蛋白 ( Fn)、层粘连蛋白 ( L aminin)、胶原( Collagen)、多聚赖氨酸和冷析蛋白 ( CIG)等 3～ 5] ,把这些因子固定到材料表面 ,可为细胞的粘附生长提供理想的条件 .本文通过碳二酰亚胺脱水缩合技术 ,将明胶 ( Gelatin)共价键合到聚甲基丙烯酸接枝改性的聚氨酯 ( PU- g- PMAA)薄膜表面 ,并初…

Immobilization of Gelatin onto Polyurethane Membrane Surface and Acceleration of Endothelial Cells Growth

Gelatin was covalently immobilized onto the polyurethane membrane surface grafted previously with poly(methacrylic acid) (PU g PMAA) using 1 (3 dimethylamino)propyl] 3 ethyl carbodiimide as the condensing reagent. ATR FTIR and ESCA characterizations verify the occurrence of the grafting, and prove that the immobilized amount of gelatin is proportional to the number of active sites, i.e . carboxylic groups on PU surface. Endothelial cells culture reveals that the cell attachment and cell growth on PU g PMAA g Gelatin are both improved obviously, demonstrating that a cell compatible PU is obtained and the immobilizing method is quite effective to introduce cell growth factors onto polymer surface.