High-throughput enzymatic method for enantiomeric excess determination of O-acetylated cyanohydrins |
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| Authors: | Hamberg Anders Lundgren Stina Penhoat Maël Moberg Christina Hult Karl |
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| Institution: | KTH School of Biotechnology, Department of Biochemistry, AlbaNova University Center, SE 106 91 Stockholm, Sweden. |
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| Abstract: | The reaction yield and enantiomeric excess of O-acetylated cyanohydrin reaction products from a library of chiral catalysts can be analyzed by a three-step screening method. Alcohol dehydrogenase and NADH are used to analyze unreacted substrate. A lipase with absolute specificity converts one enantiomer to a quantifiable product before the remaining enantiomer is hydrolyzed with an unspecific esterase and quantified. |
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