镉-钙黄绿素配合物在玻片表面的毛细流成环组装及其数值化荧光成象分析研究

一滴撒落在固载表面上的咖啡干涸后 ,会在原始液滴周边形成一个清晰的沉积环 .这种环沉积( Ring deposit)现象在其它液滴 (如牛奶、泥浆等 )干涸过程中十分普遍 .Deegan[1,2 ] 等认为这是由于发生在液滴内部的毛细流作用 ( Capillary flow effect)所致 .与电场和磁场一样 ,毛细流传输作用对溶质分子在固载表面的固定化组装具有定向作用 ( Direction effect) ,因而可以用于探针分子在固载表面的组装及其分析应用研究 [3] .同时 ,毛细流定向组装成环技术需要样品少 ,灵敏度高 ,在生化研究及分析、临床检验和环境监测方面具有十分重要的应用…

Ordered Ring Assembly of Cadmium-Calcein Complex Directed Under the Capillary Flow on the Glass Slide Supports and Its Digitalized Fluorescence Image Analysis

Afluorescent ordered ring assembly was observed under a fluorescence microscope, and the assembly image was captured by using a digital CCDcamera for analytical purposes. When a droplet of diluted solution of cadmium-calcein complex in 0.01 mol/Lhexahydropyridine was spotted onto the surface of dimethyl dichlorosilane modified glass slide, a symmetrical ordered ring was formed with an outer diameter of 1.10 mm and a ring line width of 0.048 mm. It was found that the fluorescent molecules on the ring line followed a Gaussian distribution, and with which a quantitative equation was established. For a 0.1 μLdroplet of the calcium calcein complex, the maximal fluorescence intensity of the ring is proportional to the content of calcium over the range of 3.0×10^-15～1.0×10^-13mol, and the limit of detection is 2.56×10^-16mol. The proposed method has been successfully applied to the quantification of Cd²⁺ in water samples of Jialingjiang River.