Spectrometric Studies on the Sonodynamic Damage of Protein in the Presence of Levofloxacin |
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| Authors: | Bin Liu Jun Wang Xin Wang Bing-Mi Liu Yu-Mei Kong Dan Wang and Shu-Kun Xu |
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| Institution: | (1) Department of Chemistry, Northeastern University, Shenyang, 110004, People’s Republic of China;(2) Department of Pharmacy, Liaoning University, Shenyang, 110036, People’s Republic of China;(3) Department of Chemistry, Liaoning University, Shenyang, 110036, People’s Republic of China; |
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| Abstract: | Taking bovine serum albumin (BSA) as typical molecules, the sonodynamic damage of protein in the presence of Levofloxacin
(LVFX) and its mechanism were studied by fluorescence and UV-vis spectra. Various influencing factors such as ultrasonic irradiation
time, pH value, ionic strength and solution temperature on the damage of BSA were also discussed. The results showed that
ultrasound can enhance the damage of LVFX on BSA. The damage degree of BSA was aggravated with the increase of ultrasonic
irradiation time, solution temperature and ionic strength, whereas decreased with the increase of solution pH value. Furthermore,
the reactive oxygen species (ROS) in reaction system were studied by oxidation and extraction photometry. Experimental results
showed that the amounts of superoxide anion radical (·O2
ˉ) and hydroxyl radical (·OH) were significantly more than that of singlet oxygen (1O2) in the presence of LVFX under ultrasonic irradiation. |
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