Isolation of DNA aptamers using micro free flow electrophoresis |
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Authors: | Jing Meng Bowser Michael T |
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Institution: | Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55414, USA. |
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Abstract: | A micro free flow electrophoresis (μFFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of μFFE allowed 1.8 × 10(14) sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of μFFE selection. |
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