Reversed-phase high-performance thin-layer chromatography of hematoporphyrin and its nickel,copper and zinc complexes |
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Authors: | N. Suzuki K. Saitoh Y. Sugiyama |
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Affiliation: | (1) Department of Chemistry, Faculty of Science, Tohoku University, 980 Sendai, Miyagi, Japan |
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Abstract: | Summary The thin-layer chromatographic mobility of hematoporphyrin IX (Hp) and its metal complexes on octyl (C8)- or octadecyl (C18)-bonded silica gel plate is described. The mobility order for those compounds is regular on both kinds of plate with various compositions of methanol-phosphate buffer mixtures as the developing solvents; thus, Cu-complex < Ni-complex < Hp (free acid) < Zn-complex. The combination of a C18-plate with an 85∶15 (vol/vol) mixture of methanol and phosphate buffer (pH 3) is recommended for the successful separation of these four compounds. |
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Keywords: | Thin-layer chromatography Porphyrin Metalloporphyrins Hematoporphyrin IX |
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