Synthesis of pyrrolidine-based oxy-peptide nucleic acids carrying four types of nucleobases and their transport into cytoplasm |
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Authors: | Mizuki Kitamatsu Akiko Takahashi Takashi Ohtsuki Masahiko Sisido |
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Institution: | Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama 700-0082, Japan |
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Abstract: | We synthesized 16 pyrrolidine-based oxy-peptide nucleic acid (POPNA) monomers carrying four different nucleobases onto four different stereoisomers of pyrrolidine rings. Using these monomers, we prepared POPNA oligomers, which formed sequence-specific hybrids with DNAs. The oligomer configurations influenced the hybrid stability. The oligomers were not taken into CHO cells. However, they could enter the cell cytoplasm when mixed with the influenza virus hemagglutinin peptide-arginine heptamer conjugate. |
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Keywords: | Peptide nucleic acid Solid-phase peptide synthesis Cell-penetrating peptide Confocal laser scanning microscopy |
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