光学相干成像结合反射光谱技术对小鼠烫伤模型的评估

光学相干层析成像(OCT)和反射光谱技术用于精确地评估小鼠烫伤模型.在相同作用时间不同温度下,皮肤厚度呈线性增加;皮下肌肉组织拟合吸收系数和斜率呈V字型变化,在80℃时,处在最低处;在65～85℃时,光谱一次微商的波峰与波谷明显分离,85℃之后这种能力逐渐减弱;色坐标值中,红色数值在60～70℃和90～95℃两个温度之...

Mice scald model investigated by OCT combined with reflection spectrum

OCT combined with reflective spectrum was employed to precisely evaluate the mice scald model. Under the conditions that the temperature increased by 5 degrees C from 60 to 95 degrees C with 20 s thermal effect, the cutis depth linearly increased. The fitting absorption parameters and the gradients showed the V trends. Wave peaks of first order differential were legible from 65 to 85 degrees C, then wave peaks became worse over 85 degrees C. At color coordinates, red value decreased severely from 65 to 85 degrees C and from 90 to 95 degrees C, green value continuously decreased, but blue value increased. Under the conditions that thermal effect time increased from 10 to 40 s at 90 degrees C, the main changes of above factors happened at about 20 s, and after 20 s effect time the change of these factors was weak. These factors could reflect the form and progresses of zones of coagulation, injury of cutis and hypodermis. So OCT combined with reflection spectrum could provide a novel method that can be applied for the real-time, low-cost, in vivo and noninvasive optical biopsy on scald.