首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Observation of dynamic oxygen release in hemoglobin using surface enhanced Raman scattering
Institution:1. The Blackett Laboratory, Imperial College of Science, Technology, and Medicine, Prince Consort Road, SW7 2BW London, UK;2. National Physical Laboratory, Queen’s Road, TW11 OLW Teddington, Middlesex, UK;1. School of Engineering, Edith Cowan University, 270 Joondalup Drive, Joondalup, Perth, WA 6027, Australia;2. School of Mechanical and Chemical Engineering, The University of Western Australia, 35 Stirling Highway, Crawley, Perth, WA 6009, Australia;3. Department of Medical Engineering and Physics, Royal Perth Hospital, 197 Wellington St, Perth, WA 6000, Australia;4. Can Tho University of Technology, 256 Nguyen Van Cu Street, Ninh Kieu District, Can Tho City, 900000, Vietnam;1. Department of Prosthodontics and Digital Technology, Stony Brook University School of Dental Medicine, Stony Brook, NY, USA;2. Department of Electrical and Computer Engineering, University of Hartford, West Hartford, CT, USA;3. Division of Oral and Maxillofacial Radiology, Department of Oral Health and Diagnostic Sciences, University of Connecticut School of Dental Medicine, Farmington, CT, USA;4. Division of Orthodontics, Department of Craniofacial Sciences, University of Connecticut School of Dental Medicine, Farmington, CT, USA
Abstract:Photoinduced oxygen dynamics in hemoglobin is monitored by surface enhanced resonant Raman spectroscopy (SERRS) using silver colloids. The spectra are characterized by massive transient amplifications which we assign to a charge-transfer mechanism during the opening of the heme-pockets in the release of oxygen. This transient lifts the selection rule specificity of normal Raman active modes and allows for the full density of states of the hemes to be observed. The amplification of specific forbidden modes is controlled by the orientation alignment of individual molecules in proximity with the colloid surface. We propose that the technique can be used to monitor oxygen release in single proteins.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号