| 荧光寿命测定的现代方法与应用 |
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| 引用本文： | 房喻,王辉.荧光寿命测定的现代方法与应用[J].化学通报,2001,64(10):631-636. |
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| 作者姓名： | 房喻 王辉 |
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| 作者单位： | 陕西师范大学化学系!西安710062 |
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| 基金项目： | 国家自然科学基金资助项目 (2 99730 2 4)，教育部中青年骨干教师基金资助项目 (30 0 6 ) |
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| 摘 要： | 介绍了时间相关单光子计算、相调制和频闪等三种现代荧光寿命测定方法的工作原理，指出了各种方法的优点和局限性；介绍了时间相关单光子计数实验数据的处理方法；概述了时间分辨荧光技术在化学和生命科学中的应用。
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| 关 键 词： | 荧光寿命 单光子计数 相调制法 频闪技术 测定方法 荧光物质 时间分辨荧光光谱 |
| 修稿时间： | 2001年1月31日 |
Fluorescence Lifetime Measurements: Modern Methods and Applications |
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| Fang Yu,Wang Hui.Fluorescence Lifetime Measurements: Modern Methods and Applications[J].Chemistry,2001,64(10):631-636. |
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| Authors: | Fang Yu Wang Hui |
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| Abstract: | The principles and characteristics of some of the modern techniques, including time\|correlated single\|photon counting (TCSPC),phase modulation and strobe techniques, for fluorescence lifetime measurements have been briefly introduced. The advantages and disadvantages of each method have also been pointed out. The common method used for the analysis of the fluorescence decay, taking TCSPC as an example, has been discussed in detail. On the basis of these introductions, the applications of time\|resolved fluorescence techniques in chemical and biological research have been overviewed. |
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| Keywords: | Fluorescence lifetime Time\|correlated single photon counting Phase modulation methods Strobe techniques |
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