On-bead fluorescence assay for serine/threonine kinases |
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| Authors: | Akita Shoji Umezawa Naoki Higuchi Tsunehiko |
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| Institution: | Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Nagoya, Japan. |
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| Abstract: | chemical reaction: see text]. A novel fluorescence-based assay for serine/threonine kinases is described. Base-mediated beta-elimination of the phosphate moiety and the Michael addition of a thiol-containing fluorescent molecule allows convenient and efficient detection of the enzyme activity. This approach may be broadly applicable to various serine/threonine kinases. |
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