Electrochromatography in microchips packed with conventional reversed-phase silica particles |
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Authors: | Gaspar Attila Hernandez Lilia Stevens Schetema Gomez Frank A |
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Institution: | Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA. gaspara@tigris.klte.hu |
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Abstract: | This paper shows the applicability of a disposable and inexpensive microfluidic chip for electrochromatographic separations. The chip, recently developed by us for chip-based LC, was fabricated from PDMS incorporating conventional chromatographic RP silica particles (C18) without the use of frits. Three cephalosporin antibiotics were used to demonstrate the applicability of the chip-based chromatographic packing for electrochromatographic determinations. The used sample injection method utilizes hydrodynamic pressure, thereby, reducing the propensity for sample bias during the injection. |
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