地塞米松磷酸钠和倍他米松磷酸钠两种差向异构体的FTIR,FT-Raman和SERS光谱研究

检测了地塞米松磷酸钠和倍他米松磷酸钠两种差向异构体的红外光谱(FTIR), 固体和饱和溶液的常规拉曼光谱(NRS)及以银胶为基底的表面增强拉曼光谱(SERS)。通过红外和拉曼光谱的结合，对其各基团的振动模式进行解析，并对峰进行归属和比较。结果表明：两种物质的主要区别是地塞米松磷酸钠的CH₃非对称伸缩振动和对称面内变形振动的拉曼位移较大;依据SERS作用机理和规律，推测两种物质在银胶上主要是通过P—O键、羧基和五元环与银胶垂直吸附。建立了两种差向异构体基于银纳米粒子的吸附模式，实现了对两种物质进行快速鉴别与区分的方法，为进一步研究肾上腺皮质激素类药物提供了十分重要的信息和可靠的参考。

Study of the Epimers of Dexamethasone Sodium Phosphate and Betamethasone Sodium Phosphate by FTIR, FT-Raman and SERS

Infrared spectroscopy (FTIR), normal Raman spectroscopy (NRS) of the solids and saturated solutions and surface-enhanced Raman spectroscopy (SERS) on the silver colloidal substrate were reported. The vibrational modes of each group were analyzed, and the enhanced peaks were assigned and compared. The results showed that the major differences between the epimers were the frequency of the CH₃ asymmetric stretching vibration and symmetric in-plane deformation vibration of them. According to the mechanism and laws of SERS, the epimers were absorbed on the silver colloid through the P—O bond, carboxyl group and the five-membered ring perpendicularly. The establishment of this adsorption model which realized the rapid identification and the discrimination between the epimers provided important information and useful reference of the adrenal cortical hormone drugs.