稳态光谱技术提取生物组织结构和功能互补信息研究

稳态光谱技术正被越来越多地用于组织结构和功能互补信息提取。通过仿体实验论证稳态光谱技术在光学参数和固有荧光提取方面的优越性。仿体实验中，血红蛋白被用作吸收子，聚苯乙烯小球被用作散射子，Stilbene被用来产生荧光。实验总共制备两组仿体，它们具有相同的散射系数和不同的吸收系数，在每组仿体中进行4次荧光素滴定。实验结果表明，组织光学参数和固有荧光均能够被精确提取，从而证明了稳态光谱技术在提取具有互补特性的组织结构和功能信息方面的优越性。

Steady-State Spectroscopy on Complementary Tissue Structural and Functional Information Extraction

Steady-state spectroscopy is prevailing for complementary tissue structural and functional information extraction. The goal of the present study is to experimentally validate the merit of this technique for optical properties and intrinsic fluorescence extraction from tissue mimicking phantoms. Phantom studies were carried out in which HbO₂ was used as absorber, polystyrene sphere was used as scatterer, and stilbene was used for fluorescence emission. Two sets of phantoms corresponding to two absorption levels and one scattering level were made, and 4 fluorophore concentrations were titrated in each set. The results indicate that both optical properties and intrinsic fluorescence can be extracted with high accuracy, implying that it’s feasible to extract complementary structural and functional information with steady-state spectroscopy.