Detection of flavonoids and assay for their antioxidant activity based on enlargement of gold nanoparticles |
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Authors: | Jing Wang Nandi Zhou Zhiqiang Zhu Junyi Huang Genxi Li |
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Institution: | (1) Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, China;(2) School of Life Science and Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University, Shanghai, 200444, China |
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Abstract: | We report our findings that natural flavonoids such as quercetin, daizeol and puerarin can act as reductants for the enlargement
of gold nanoparticles (Au-NPs). Consequently, the UV–vis spectra of a solution containing Au-NPs will be gradually changed,
and the molecules of the natural herbs can be detected by making use of changes in the UV–visible spectra. Furthermore, we
have prepared a self-assembled monolayer modified electrode by modifying cysteamine on a gold substrate electrode, which is
further modified by some Au-NP seeds. When the modified electrode is immersed in a solution containing flavonoids and tetrachloroauric
acid as a gold source for the growth of the Au-NP seeds, with the increase of the concentration of flavonoids, the Au-NP seeds
on the surface of the modified electrode can be enlarged to varying degrees. As a result, the peak currents in the corresponding
cyclic voltammograms are inversely decreased, and simultaneously the peak separation is increased. Therefore, an electrochemical
method to detect flavonoids is also proposed. Compared with the optical detection method, the electrochemical method has an
extraordinarily lower detection limit and a significantly extended detection range. Moreover, the optical and electrochemical
experimental results can be also used to assay and compare the relative antioxidant activities of the flavonoids.
Figure Enlargement of Au nanoparticles by flavonoids at cysteamine modified electrode |
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Keywords: | Gold nanoparticles Flavonoid Antioxidant Detection |
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