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Integrated microfluidic platform for the electrochemical detection of breast cancer markers in patient serum samples
Authors:Fragoso Alex  Latta Daniel  Laboria Noemi  von Germar Frithjof  Hansen-Hagge Thomas E  Kemmner Wolfgang  Gärtner Claudia  Klemm Richard  Drese Klaus S  O'Sullivan Ciara K
Institution:Departament d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona, Spain. alex.fragoso@urv.cat
Abstract:A microsystem integrating electrochemical detection for the simultaneous detection of protein markers of breast cancer is reported. The microfluidic platform was realized by high precision milling of polycarbonate sheets and features two well distinguishable sections: a detection zone incorporating the electrode arrays and the fluid storage part. The detection area is divided into separate microfluidic chambers addressing selected electrodes for the measurement of samples and calibrators. The fluidic storage part of the platform consists of five reservoirs to store the reagents and sample, which are interfaced by septa. These reservoirs have the appropriate volume to run a single assay per cartridge and are manually filled. The liquids from the reservoirs are actuated by applying a positive air pressure (i.e.via a programmable syringe pump) through the septa and are driven to the detection zone via two turning valves. The application of the realised platform in the individual and simultaneous electrochemical detection of proteic cancer markers with very low detection limits are demonstrated. The microsystem has also been validated using real patient serum samples and excellent correlation with ELISA results obtained.
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