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Determination of Triandrin and Salicin in Salix viminalis L. by Reversed-Phase High-Performance Liquid Chromatography |
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| Authors: | R A Minakhmetov L A Onuchak V A Kurkin G G Zapesochnaya S A Medvedeva |
| Institution: | (1) Department of Chemistry, Samara State University, ul. akademika Pavlova 1, Samara, 443011, Russia;(2) Department of Pharmacology, Samara State Medical University, ul. Chapaevskaya 89, Samara, 443099, Russia |
| Abstract: | The optimal separation of phenolic components of the bark of basket willow Salix viminalis L. was attained using reversed-phase high-performance liquid chromatography with isocratic elution. Triandrin (1-O- -D-glucopyranoside of p-coumaryl alcohol) and salicin (1-O--D-glucopyranoside of salicyl alcohol) were identified. Regularities in the retention of triandrin and salicin in the Separon SGX C18–binary mobile phase (acetonitrile–water) system were considered. A procedure was developed for the quantitative determination of triandrin in raw basket-willow bark and its extract. |
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