Photoregulation of DMA Photolyases in Broom Sorghum Seedlings |
| |
| Authors: | Megumi Hada Günther Buchholz Tohru Hashimoto Osamu Nikaido Eckard Wellmann |
| |
| Institution: | Department of Biology, Faculty of Science, Kobe University, Kobe, Japan;Institute of Biology II, Freiburg University, Freiburg, Germany;Department of Life Science, Kobe Women's University, Kobe, Japan;Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Japan |
| |
| Abstract: | Abstract— Using dark-grown 3 day-old Sorghum bicolor seedlings, photorepair of cyclobutane pyrimidine dimers (CPD) by CPD-photoIyase and pyrimidine-(6–4)pyrimidinone photoproducts (6–4]PP) by 6–4]PP-photolyase was studied in vivo and in vitro. The two types of DNA photoproducts were measured with specific monoclonal antibodies. Sorghum seedlings have different photorepair enzymes for CPD and 6–4]PP. The CPD-photolyase was found to be increased in response to light with maximal efficiency in the UVA/blue spectral range, whereas 6–4]PP-photolyase was not influenced by light. |
| |
| Keywords: | |
|
|
