The application of retention indices using the alkylarylketone scale to the separation of the barbiturates by HPLC. I. The effect of the eluent |
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| Authors: | Roger M Smith Tony G Hurdley Richard Gill Anthony C Moffat |
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| Institution: | (1) Department of Chemistry, University of Technology, LE11 3TU Loughborough, Leics, U.K.;(2) Central Research Establishment, Home Office Forensic Science Service, RG7 4PN Aldermaston, Reading, U.K. |
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| Abstract: | Summary In order to determine the applicability of retention indices, based on the alkylarylketone scale, as the basis of a reproducible
method of reporting retentions, the separation of ten barbiturates and a set of column test compounds were examined on an
octadecylsilyl bonded silica (ODS-Hypersil) column with methanol-buffer pH 8.5 as eluent. The effects on the capacity factors
and retention indices, on changing the eluent composition, pH, ionic strength and temperature, showed that the retention indices
of the barbiturates were much less susceptible to minor changes in the eluent than the capacity factors. For non-ionised compounds
the retention indices were virtually independent of the experimental conditions.
Presented at the 15th International Symposium on Chromatography, Nürnberg, October 1984 |
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| Keywords: | Retention indices Liquid chromatography Barbiturates Alkylarylketones Reproducibility |
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