Insertion of an unnatural amino acid into the protein structure: preparation and properties of 3-fluorotyrosine-containing organophosphate hydrolase |
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| Authors: | Yu A Votchitseva E N Efremenko S D Varfolomeyev |
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| Institution: | (1) Department of Chemistry, M. V. Lomonosov Moscow State Univertsity, 1 Leninskie Gory, 119992 Moscow, Russian Federation |
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| Abstract: | Five Tyr residues present in the native organophosphate hydrolase (OPH) containing a hexahistidine tag at the N-terminus of
the protein molecule (His6-OPH) were replaced by fluorine-containing analogs using a biosynthetic approach. The modified enzyme had an extended pH-optimum
of action shifted to acidic pH and an enhanced thermal stability in the alkaline pH region.
Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 2, pp. 357–361, February, 2006. |
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| Keywords: | 3-fluorotyrosine organophosphate hydrolase unnatural amino acid paraoxon |
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