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Synthesis and Characterization of a Biocompatible Copolymer to be Used as Cell Culture Support |
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| Authors: | Natalia Becerra Luz Marina Restrepo Betty Lucy López |
| Affiliation: | 1. Grupo Ciencia de los Materiales, Universidad de Antioquia, Calle 62 N° 52 59 Medellín, Colombia Grupo de Ingeniería de Tejidos y Terapias Celulares, Universidad de Antioquia, Calle 62 52 59 Medellín, Colombia Fax: (+574) 210 6550;2. Grupo de Ingeniería de Tejidos y Terapias Celulares, Universidad de Antioquia, Calle 62 52 59 Medellín, Colombia Fax: (+574) 210 6550;3. Grupo Ciencia de los Materiales, Universidad de Antioquia, Calle 62 N° 52 59 Medellín, Colombia |
| Abstract: | Summary: N-isopropylacrylamide (NIPAAm)/butylacrylate (BAc) copolymer was synthesized by emulsion polymerization in order to use it as a cell culture surface for corneal epithelium biosubstitutes. Results showed that the obtained polymers were thermosensitive hydrogels. The copolymer was characterized by Fourier transform infrared (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and swelling degree. 3T3 Swiss cells were used as feeder layers and they were used to test the cytotoxic effect of the hydrogels. The conditions to isolate, cultivate, expand and cryo-preserve human oral mucosal cells were established and analysis of several morphological and immuno-histochemical characteristics of the cultivated oral mucous were made. |
| Keywords: | biocompatibility biomaterials hydrogels stimuli-sensitive polymers tissue engineering |