| 纳米脂质体包裹荧光试剂进入单细胞的研究 |
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| 引用本文： | 孙悦,卢敏,殷学锋. 纳米脂质体包裹荧光试剂进入单细胞的研究[J]. 高等学校化学学报, 2006, 27(4): 632-634 |
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| 作者姓名： | 孙悦 卢敏 殷学锋 |
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| 作者单位： | 浙江大学化学系微分析系统研究所;浙江大学生命科学学院生物大分子与酶工程研究所,杭州,310027 |
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| 摘 要： | 本研究首次使用直径约100 nm的小脂质体包裹荧光染料, 通过细胞的内吞作用或融合过程, 转移不透膜荧光物质进入细胞内, 标记细胞内组分.
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| 关 键 词： | 单细胞分析 脂质体 细胞内衍生 荧光染料 |
| 文章编号： | 0251-0790（2006）04-0632-03 |
| 收稿时间： | 2005-12-20 |
| 修稿时间： | 2005-12-20 |
Intracellular Delivery of Fluorescent Dyes Mediated by Nanometer-Liposomes |
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| SUN Yue,LU Min,YIN Xue-Feng. Intracellular Delivery of Fluorescent Dyes Mediated by Nanometer-Liposomes[J]. Chemical Research In Chinese Universities, 2006, 27(4): 632-634 |
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| Authors: | SUN Yue LU Min YIN Xue-Feng |
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| Affiliation: | 1. Institute of Microanalytical Systems, Department of Chemistry; 2. Institute of Biologic Macromolecule and Enzymatic Engineering, College of Life Science, Zhejiang University, Hangzhou 310027, China |
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| Abstract: | A new method that nanometer-liposomes could penetrate the cell membrane to deliver their encapsulated material into cells was investigated.Nanometer sized-liposomes were produced from phosphatidylcholine to encapsulate fluorescent dyes and FITC labeled superoxide dismutase(SOD-FITC) by an ultrasonic method.The size distribution of liposomes was determined with a Laser Particle Analyser.The fluorescence images demonstrate that liposomes can transfer fluorescent dyes(FITC,Rhodamine B),which are not cell membrane permeable,into cells.Single cell analysis of the delivered SOD-FITC with microchip electrophoresis revealed that the density of liposomes and interaction-time between liposomes and cells affected the efficiency of intracellular delivery of encapsulated material. |
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| Keywords: | Single cell analysis Liposome Intracellular derivatization Fluorescent dye |
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