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Observation of subcellular nanostructure of single neurons with an illumination mode photon scanning tunneling microscope |
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| Authors: | R Uma Maheswari H Tatsumi Y Katayama M Ohtsu |
| Institution: | a Kanagawa Academy of Science and Technology, KSP East 408, 3-2-1 Sakado, Takatsu-Ku, Kawasaki-Shi, Kanagawa 213, Japan b Department of Autonomic Physiology, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda-Ku, Tokyo 101, Japan c PRESTO, Research Development Corporation of Japan, City, Japan d Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-Ku, Yokohama, Kanagawa 226, Japan |
| Abstract: | We report about the observation of microtubules lying underneath the cell membrane of neural process in neurons with a resolution as high as that of an electron microscope by an illumination mode photon scanning tunneling microscope. Nanoapertures used in our observations were fabricated by means of selective chemical etching and metal coating of an optical fiber. The narrowest observed tube has got an average diameter of 26 nm. Comparing this with its nominal value of 25 nm, the difference which is considered as a measure of resolution (δ) is 1 nm implying a resolution comparable to that of an electron microscope in imaging dielectric specimens. This was possible due to the presence of a boundary between the glass and the metal coating and also due to the use of an aperture of almost the same size as that of the microtubule that enhances the detection. |
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