High-performance liquid chromatographic method for the determination of alprazolam in plasma using the column-switching technique. |
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| Authors: | W Rieck D Platt |
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| Affiliation: | Lehrstuhl für Innere Medizin, Gerontologie, Universit?t Erlangen-Nürnberg, Germany. |
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| Abstract: | A reversed-phase high-performance liquid chromatographic method is described for the quantitative determination of alprazolam in the plasma of geriatric patients in the presence of 4-hydroxyalprazolam, alpha-hydroxyalprazolam, bromazepam, oxazepam, lorazepam, clobazam, desmethylclobazam, diazepam and desmethyldiazepam. The procedure is based on the enrichment of alprazolam on a PRP-1 pre-column, followed by the transfer of the compound in a forflush mode to the analytical column. Alprazolam can be quantified reliably down to a minimum concentration of 1 ng/ml of plasma. |
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