| 水溶性荧光聚合物用于测定聚赖氨酸 |
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| 引用本文： | 陈彦国,何治柯. 水溶性荧光聚合物用于测定聚赖氨酸[J]. 高等学校化学学报, 2005, 26(8): 1428-1431 |
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| 作者姓名： | 陈彦国 何治柯 |
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| 作者单位： | 1. 武汉大学化学与分子科学学院, 武汉430072;2. 湖北师范学院化学系, 黄石435002 |
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| 基金项目： | 国家自然科学基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 合成了一种水溶性荧光聚合物聚[5-甲氧基-2-(3-磺酰化丙氧基)-1,4-苯撑乙烯](MPS-PPV),对该聚合物进行了元素分析、红外光谱及透射电子显微镜表征.实验结果表明,聚赖氨酸(PLL)对该聚合物荧光具有明显的猝灭作用,据此建立了一种快速灵敏测定聚赖氨酸的新方法,检出限为5.0×10-10mol/L.探讨了聚赖氨酸猝灭MPS-PPV荧光的机理.
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| 关 键 词： | 荧光 聚合物 聚[5-甲氧基-2-(3-磺酰化丙氧基)-1 4-苯撑乙烯] 聚赖氨酸 |
| 文章编号： | 0251-0790（2005）08-1428-04 |
| 收稿时间： | 2004-09-20 |
| 修稿时间： | 2004-09-20 |
Determination of Poly-L-lysine with a Water-soluble Fluorescence Polymer |
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| CHEN Yan-guo,He Zhi-ke. Determination of Poly-L-lysine with a Water-soluble Fluorescence Polymer[J]. Chemical Research In Chinese Universities, 2005, 26(8): 1428-1431 |
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| Authors: | CHEN Yan-guo He Zhi-ke |
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| Affiliation: | 1. College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China;2. Department of Chemistry, Hubei Normal University, Huangshi 435002, China |
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| Abstract: | A water-soluble fluorescence polymer poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylene-vinylene](MPS-PPV) was synthesized and characterized by elemental analysis, infrared absorption spectrun and the transmission electron microscope micrograph. The fluorescence of MPS-PPV could be quenched by poly-L-lysine(PLL), a new food preservative. So a rapid and sensitive method for the determination of PLL was set up, the linear range and detection limit were 3.6×10-92.5×10-8 mol/L and 5.0×10-10 mol/L respectively. The fluorescence quenching mechanism of PLL to MPS-PPV was also discussed, which was caused by the strong electrostatic interaction between cationic PLL and anion conjugated polymer MPS-PPV. |
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| Keywords: | Fluorescence Polymer Poly[5-methoxy-2-(3-sulfopropoxy)-1 4-phenylenevinylene] Poly-L-lysine |
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