Evidence for the preservation of specific intermolecular interactions in gaseous protein-oligosaccharide complexes |
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Authors: | Kitova Elena N Bundle David R Klassen John S |
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Affiliation: | Department of Chemistry, University of Alberta, Edmonton, Canada T6G 2G2. |
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Abstract: | Arrhenius parameters, obtained with the blackbody infrared radiative dissociation technique, are reported for the dissociation of a series of gaseous protonated complexes composed of mutants of a single chain variable fragment (scFv) of the monoclonal antibody Se155-4 and three trisaccharide ligands. Hydrogen bonding between a ligand and a particular amino acid residue is identified from a comparison of activation energies measured for the complex of the unmodified scFv and the corresponding mutant. It is shown that the specific hydrogen bond between His(101H) and Man C-4 OH is preserved in the gaseous complex. |
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