Analytical selenoamino acid studies by chromatography with interfaced atomic mass spectrometry and atomic emission spectral detection |
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Authors: | P C Uden Susan M Bird Mihaly Kotrebai Paula Nolibos Julian F Tyson Eric Block and Eric Denoyer |
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Institution: | (1) Department of Chemistry, Lederle Graduate Research Tower A, University of Massachusetts, Box 35410, Amherst, MA 01003-4510, USA, US;(2) Department of Chemistry, SUNY-Albany, Albany, NY 12222, USA, US;(3) The Perkin-Elmer Corporation, 761 Main Ave., Norwalk, CT 06859, USA, US |
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Abstract: | Consumption of selenium enriched plants or yeast-based nutritional supplements has been reported to provide anticarcinogenic
benefits which are selenium compound dependent. Separation and identification of these selenium compounds is critical to understand
the activity. Plants and yeast convert inorganic selenium in the soil or growth media into organoselenium compounds, probably
following a route similar to the sulfur assimilatory pathway. Non-volatile selenium compounds produced include selenoamino
acids, some of which have shown anticarcinogenic activity. Volatile compounds produced by chemical reaction of involatile
precursors have also been found. An ion pair chromatographic method with ICP-MS detection for the separation of selenoamino
acid standards potentially present in real samples is given. The method allows separation of selenoamino acids including such
analytes as the cis-trans isomers of Se-1-propenyl-dl-selenocysteine. The method also provides the capability of determining the presence of selenoxides and possibly selenones,
and tracking of other functionalities and reactions by selective derivatization. Alternatively, selenoamino acids are treated
with ethylchloroformate to produce stable volatile derivatives which are amenable to GC separation with element specific atomic
emission detection (GC-AED). Results of total selenium determination and speciation of selenium enriched yeast-based nutritional
supplements, selenium enriched allium vegetables and bioremediation samples are presented.
Received: 16 February 1998 / Revised: 4 June 1998 / Accepted: 9 June 1998 |
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