HPLC separation of catecholamines after derivatization with 9-fluorenylmethyl chloroformate |
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| Authors: | A. A. Descombes W. Haerdi |
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| Affiliation: | (1) Department of Inorganic, Analytical and Applied Chemistry, Sciences II, University of Geneva, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland |
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| Abstract: | Summary Optimum conditions for the separation of 9-fluorenylmethyl chloroformate derivatized catecholamines by HPLC are described; three catecholamines (noradrenaline, adrenaline and dopamine) and an internal standard (epinine) were separated in less than 20 minutes under isocratic conditions. This method is 17 to 350 times more sensitive than electrochemical detection, depending on the test compounds. It has been applied to the analysis of catecholamines in urine. The sample was extracted by a metal-loaded silica prior to separation. |
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| Keywords: | Column liquid chromatography Pre-derivatization Catecholamines 9-fluorenylmethyl chloroformate (FMOC-Cl) |
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