Circulating hemocytes from larvae of Melipona scutellaris (Hymenoptera,Apidae, Meliponini): Cell types and their role in phagocytosis |
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| Authors: | Isabel Marques Rodrigues Amaral Jo?o Felipe Moreira Neto Gustavo Borges Pereira Mariani Borges Franco Marcelo Emílio Beletti Warwick Estevam Kerr Ana Maria Bonetti Carlos Ueira-Vieira |
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| Institution: | 1. Laboratório de Genética, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Campus Umuarama, Sala 2E33, Uberlândia-MG, CEP: 3840-902, Brazil;2. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil;3. Instituto de Ciências Biomédicas, Laboratório de Histologia, Universidade Federal de Uberlândia-MG, Brazil;1. Université de Lorraine, Inra, LSE, F-54000 Nancy, France;2. Université de Lorraine, IUT de Thionville-Yutz, F-57970 Yutz, France;1. Department of Animal Reproduction, School of Veterinary Medicine, UNIRP – Centro Universitário de Rio Preto, Unidade Universitária II (Hospital Veterinário), Rodovia Br 153 (Transbrasiliana), Km 69, 15093-450, São José do Rio Preto, SP, Brazil;2. Department of Preventive Veterinary Medicine and Animal Reproduction, School of Agrarian Sciences and Veterinary Medicine, Univers Estadual Paulista, Jaboticabal, SP 14884-900, Brazil;3. School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP 13630-000, Brazil;4. School of Animal Science, Michigan State University, East Lansing, MI 48824, USA;5. School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, MG 38400-902, Brazil;1. Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, Pernambuco, Brazil;2. Departamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães – (CPqAM/FIOCRUZ), Recife, Pernambuco, Brazil;3. Departamento de Fisiologia e Patologia, Universidade Federal da Paraíba (UFPB), João Pessoa, Brazil;4. Departamento de Ciências Fisiológicas, Universidade de Pernambuco (UPE), Recife, Pernambuco, Brazil;5. Laboratório de Imunopatologia Prof°. Keizo Asami (LIKA) da Universidade Federal de Pernambuco, Recife, PE, Brazil;1. Department of Biology, Ecology and Earth Science, University of Calabria, Italy;2. Department of Life Sciences, University of Trieste, Italy |
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| Abstract: | Infection in insects stimulates a complex defensive response. Recognition of pathogens may be accomplished by plasma or hemocyte proteins that bind specifically to bacterial or fungal polysaccharides. Several morphologically distinct hemocyte cell types cooperate in the immune response. Hemocytes attach to invading organisms and then isolate them by phagocytosis, by trapping them in hemocyte aggregates called nodules, or by forming an organized multicellular capsule around large parasites. In the current investigation the cellular in the hemolymph third instar larvae of M. scutellaris has been characterized by means of light microscopy analysis and phagocytosis assays were performed in vivo by injection of 0.5 μm fluorescence beads in order to identify the hemocyte types involved in phagocytosis. Four morphotypes of circulating hemocytes were found in 3rd instar larvae: prohemocytes, plasmatocytes, granulocytes and oenocytoids. The results presented plasmatocytes and granulocytes involved in phagocytic response of foreign particles in 3rd instar larvae of M. scutellaris. |
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