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Characterisation of individual pixel efficiency in the PILATUS II sensor
Authors:A Schubert  GJ O’Keefe  BA Sobott  NM Kirby  RP Rassool
Institution:1. School of Physics, University of Melbourne, Parkville, 3010, Australia;2. Australian Synchrotron, Clayton, Victoria 3168, Australia;3. CRCBID Cooperative Research Centre for Biomedical Imaging, Bundoora, Victoria 3083, Australia;4. Centre for PET, Austin Hospital, Heidelberg, Victoria 3084, Australia;1. Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada;3. State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China;4. The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, China;5. the Xiamen City Key Laboratory of Biliary Tract Diseases, Xiang''an Hospital of Xiamen University, Xiamen, Fujian 361101, China;6. Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030;3. Department of Pathology, Division of Microbiology and Immunology, University of Utah School of Medicine, Salt Lake City, Utah 84132;4. DNA Sequencing Core, University of Utah School of Medicine, Salt Lake City, Utah 84132;5. Department of Biology, Indiana University-Purdue University, Indianapolis, Indiana 46202;6. Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112;1. Biomedical Engineering, Vanderbilt University, Nashville, Tennessee;2. Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri;1. Department of Mechanical Engineering, University of Washington, Seattle, Washington;2. Division of Hematology, Department of Medicine, University of Washington, Seattle, Washington;3. Department of Biochemistry, University of Washington, Seattle, Washington;4. Department of Bioengineering, University of Washington, Seattle, Washington;5. Institute of Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington;6. BloodWorks Northwest Research Institute, Seattle, Washington;3. Laboratory of Molecular and Cellular Biology, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto 606-8507;4. Medical Research Support Center, Graduate School of Medicine, Kyoto University, Kyoto 606-8501;5. Department of Cell Sciences, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
Abstract:Synchrotron applications such as protein crystallography and small-angle X-ray scattering (SAXS) demand precise knowledge of detector pixel efficiency for data corrections. Current techniques used to determine detector efficiency are only applicable for the specific set-up for which the calibration is performed. Here the effect of comparator thresholding on pixel efficiency for PILATUS is presented for standard amplifier and shaper gain settings, allowing users to make necessary corrections to their intensity data for various threshold settings without requiring repeated empirical calibrations. A three-dimensional TCAD simulation of the sensor is also presented and is used to confirm the experimental result.
Keywords:
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