Localisation of hydrogen peroxide accumulation during Solanum tuberosum cv. Rywal hypersensitive response to Potato virus Y |
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| Authors: | Katarzyna Otulak Gra?yna Garbaczewska |
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| Affiliation: | 1. Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA;2. Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA;1. City of Hope Comprehensive Cancer Center, Duarte, CA;2. Analysis Group Inc, Boston, MA;3. Novartis Pharmaceuticals Corporation, East Hanover, NJ;4. US Oncology Research, Comprehensive Cancer Centers of Nevada, Las Vegas, NV;1. School of Pharmacy, College of Medicine, National Taiwan University, 1, Section 1, Jen-Ai Road, Taipei 100, Taiwan;2. Division of Silviculture, Taiwan Forestry Research Institute, Taipei 100, Taiwan;3. School of Pharmacy, Taipei Medical University, Taipei 110, Taiwan;4. National Research Institute of Chinese Medicine, Taipei 112, Taiwan;5. Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan;1. Centro Nacional de Biotecnología-CSIC, Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain;2. Instituto de Biología Molecular y Celular de las Plantas (CSIC-Universidad Politécnica de Valencia), Avenida de los Naranjos s/n, 46022 Valencia, Spain |
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| Abstract: | The reactive oxygen species hydrogen peroxide (H2O2) was detected cytochemically in Solanum tuberosum cv. Rywal tissues as a hypersensitive response (HR) 24 and 48 h after a Potato virus Y (PVY) infection.Hydrogen peroxide was detected in vivo by its reaction with 3.3-diaminobenzidine, producing a reddish-brown staining in contact with H2O2. Hydrogen peroxide was detected in the necrotic area of the epidermal and mesophyll cells 24 and 48 h after the PVY infection. Highly localised accumulations of H2O2 were found within xylem tracheary elements, and this was much more intensive than in non-infected leaves. Hydrogen peroxide was detected cytochemically in HR also by its reaction with cerium chloride, producing electron-dense deposits of cerium perhydroxides.Inoculation with PVYNTN and also PVYN Wi induced a rapid hypersensitive response during which highly localised accumulations of H2O2 was detected in plant cell walls. The most intensive accumulation was present in the bordering cell walls of necrotic mesophyll cells and the adjacent non-necrotic mesophyll cells. Intensive electron-dense deposits of cerium perhydroxide were found along ER cistrenae and chloroplast envelopes connected with PVY particles. The precipitates of hydrogen peroxide were detected in the nuclear envelope and along tracheary elements, especially when virus particles were present inside. The intensive accumulation of H2O2 at the early stages of potato–PVY interaction is consistent with its role as an antimicrobial agent and for this reason it has been regarded as a signalling molecule. |
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