The first cytogenetic characterization of the poisonous black widow spider Latrodectus gr. curacaviensis from Brazil,with chromosomal review of the family Theridiidae (Arachnida,Araneae) |
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| Authors: | Douglas Araujo Ulysses Madureira Maia Antonio Domingos Brescovit |
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| Institution: | 1. Universidade Estadual de Mato Grosso do Sul – UEMS, Unidade Universitária de Mundo Novo, BR 163, Km 20.2, Bairro Universitário, CEP 79980-000, Mundo Novo, Mato Grosso do Sul, Brazil;2. Universidade do Estado do Rio Grande do Norte – UERN, Faculdade de Ciências Exatas e Naturais – FANAT, Departamento de Ciências Biológicas, DECB, Campus Universitário Central, BR 110, Km 48, Rua Professor Antônio Campos, s/n, Bairro Costa e Silva, CEP 59610-090, Mossoró, Rio Grande do Norte, Brazil;3. Instituto Butantan, Laboratório de Artrópodes, Av. Vital Brazil, 1500, CEP 05503-900, São Paulo, São Paulo, Brazil;1. Institute of Physiology & Biophysics, Uzbek Academy of Sciences, Tashkent, Uzbekistan;2. Cardiovascular Therapeutics Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria 3010, Australia;3. School of Medical & Molecular Biosciences, University of Technology, Sydney, NSW 2007, Australia;4. Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria 3010, Australia;1. Área Investigación y Desarrollo-Venenos, Instituto Nacional de Producción de Biológicos, Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”, Ministerio de Salud de la Nación, Argentina;2. Primera Cátedra de Toxicología, Facultad de Medicina, Universidad de Buenos Aires, Argentina;3. Laboratorio de Toxinopatología, Centro de Patología Experimental y Aplicada, Facultad de Medicina, Universidad de Buenos Aires, Argentina;4. Departamento de Parasitología, Instituto Nacional de Enfermedades infecciosas, Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”, Ministerio de Salud de la Nación, Argentina;5. Centro de Estudios Parasitológicos y de Vectores (CEPAVE)/CONICET, Universidad Nacional de La Plata, Argentina;6. Laboratorio Central de Salud Pública, Ministerio de Salud de la Provincia de Buenos Aires, Argentina;7. Cátedra de Inmunología II, Microbiología Clínica e Industrial, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina;8. Departamento de Zoonosis, Ministerio de Salud de la Provincia de Catamarca, Argentina;9. Departamento de Investigación, Secretaría Salud de la Provincia de Chubut, Argentina;10. Escuela de Veterinaria, Universidad Nacional de Río Negro, Provincia de Rio Negro, Argentina;11. Dirección de Salud Ambiental, Ministerio de Salud de la Provincia de Rio Negro, Argentina;12. Instituto de Animales Venenosos “Jorge Washington Ábalos”, Ministerio de Salud de la Provincia de Santiago del Estero, Argentina;13. Dirección Nacional de Determinantes de la Salud e Investigación, Ministerio de Salud de la Nación, Argentina;1. Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, BA, Brazil;2. Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brazil;3. Instituto Gonçalo Moniz FIOCRUZ, Salvador, BA, Brazil;4. Instituto Vital Brasil, Niterói, RJ, Brazil;5. Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;6. Centro de Biotecnologia e Terapia Celular, Hospital São Rafael, Salvador, BA, Brazil |
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| Abstract: | In this paper we present, for the first time, cytogenetical data on Latrodectus gr. curacaviensis (Theridiidae) from Brazil, as well as the first data on meiosis and sex chromosome system of this genus. Testes were submitted to colchicine, hypotonic, and fixation treatment, and chromosomal preparations were stained with Giemsa solution. The analysis showed 2n = 26 telo/acrocentric chromosomes in spermatogonial metaphases. Metaphase I exhibited 12 autosomal bivalents and two sex chromosome univalents (12II + X1X2). All bivalents revealed one terminal chiasma. Metaphases II confirmed the sex chromosome system, showing 12 autosomes or 12 autosomes plus two X chromosomes, respectively. Male karyotype prevailing in theridiids is formed by 2n = 22 chromosomes, including sex chromosome system X1X2 in all species. The Latrodectus species of the geometricus clade analyzed until now showed smaller diploid number (2n♀ = 16 and 2n♀ = 18) than the species of the mactans clade (2n♀ = 24 and 2n♀ = 26). Thus, according to the chromosome number, the examined Latrodectus species seems to be related to the mactans clade. |
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