An Equilibrium and a Kinetic Stopped-Flow Fluorescence Study of the Binding of Various Metal Ions to Goat Alpha-Lactalbumin |
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Authors: | H. Van Dael A. Chedad |
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Affiliation: | (1) Interdisciplinary Research Centre, K.U. Leuven Campus Kortrijk, Sabbelaan 53, 8500 Kortrijk, Belgium |
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Abstract: | Various metal ions bind to the protein α-lactalbumin prepared from goat milk. The stability of the protein after metal binding is compared with that of the apo-protein by monitoring the fluorescence of the tryptophan residues under equilibrium conditions. The kinetics of the metal binding is studied by stopped-flow fluorescence spectroscopy. By means of the Arrhenius plots, the activation energy with regard to the binding of the different ions is determined. |
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Keywords: | Metal binding α -Lactalbumin Binding kinetics Fluorescence spectroscopy |
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