Mass spectrometric analysis of nanoscale sample volumes extracted from open microchannels after sample preconcentration applied on amyloid beta peptides |
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Authors: | Saara Mikkonen Johan Jacksén Åsa Emmer |
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Affiliation: | 1. Department of Chemistry, Division of Applied Physical Chemistry, Analytical Chemistry, School of Chemical Science and Engineering, KTH Royal Institute of Technology, Teknikringen 36, 100 44, Stockholm, Sweden
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Abstract: | A new instrumental concept for extraction of nanovolumes from open microchannels (dimensions 150 μm?×?50 μm, length 10 mm) manufactured on silicon microchips has been used in combination with a previously developed method for preconcentrating proteins and peptides in the open channels through electromigration. The extracted nanovolumes were further analyzed using nanoelectrospray ionization (nESI) or matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) directly or with subsequent enzymatic protein digestion in a nanodroplet prior to the MS analysis. Preconcentration of the samples resulted in a 15-fold sensitivity increase in nESI for a neurotensin solution, and using MALDI-MS, amyloid beta (Aβ) peptides could be detected in concentrations down to 1 nM. The method was also successfully applied for detection of cell culture Aβ. |
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