Abstract: | A laccase that has been isolated previously (1) from the Basidiomycete,Rigidoporus lignosus, a white rot fungus of rubber tree, was used in the present study. When a thioglycolic lignin (TGL) was incubated in the
presence of this enzyme, pronounced changes in the UV spectrum and size distribution of the substrate were observed. Sephadex
gel filtration indicated that two types of reactions occurred: (1) A degradation of the polymer, as evidenced by the production
of low-molecular-weight material; and (2) a condensation of some of the TGL molecules, as revealed by an increase in the fractions
of higher molecular weight. |