Capillary electrophoresis determination of carnitine in food supplements |
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Authors: | Prokorátová Vendula Kvasnicka Frantisek Sevcík Rudolf Voldrich Michal |
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Institution: | Institute of Chemical Technology, Department of Food Preservation and Meat Technology, Technicá 5, 166 28 Prague 6, Czech Republic. |
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Abstract: | L-Carnitine is a substance natural for human body which transfers fatty acids to the place of burning-mitochondria and aids the transformation of fats into energy and this way supports overweight reduction and immediate physical performance, increases resistance from physical load and protect heart from overload. In this study are described newly developed electrophoretic methods (ITP, CZE with direct and/or indirect UV detection) for carnitine determination in various samples. The results were compared with results obtained by validated HPLC method. All of these methods gave comparable results. The detection limits of the electrophoretic methods were between 2.4 and 4.7 microg/ml, reproducibility (relative standard deviation, RSD%) was between 1.2 and 4.4% and recoveries were between 91 and 113% in different samples. The shorter analysis and low running cost are the main advantages of CE methods. |
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Keywords: | Carnitine Electrophoresis ITP CZE Food supplements |
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