Separation of proteins using supramolecular gel electrophoresis |
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Authors: | Yamamichi Sachiyo Jinno Yuki Haraya Nana Oyoshi Takanori Tomitori Hideyuki Kashiwagi Keiko Yamanaka Masamichi |
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Institution: | Department of Chemistry, Faculty of Science, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan. |
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Abstract: | An amphiphilic low-molecular-weight hydrogelator 1 was synthesized. A tris-glycine-SDS solution gel of 1 was applied for electrophoresis to separate proteins. Centrifugation of a mixture of protein and a hydrogel of 1 enabled the recovery of protein. Various combinations of proteins were applied for supramolecular gel electrophoresis (SUGE), and remarkably poor mobility for small proteins (<45 kDa) was found. |
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