|
|||||
|
|
Recent advances in proteolysis and peptide/protein separation by chromatographic strategies |
|
| Authors: | ZHANG XiangMin LIU BaoHong ZHANG LiHua ZOU HanFa CAO Jing GAO MingXia TANG Jia LIU Yun YANG PengYuan & ZHANG YuKui |
| Affiliation: | ZHANG XiangMin1,LIU BaoHong1,ZHANG LiHua2,ZOU HanFa2,CAO Jing1,GAO MingXia1,TANG Jia1,LIU Yun1,YANG PengYuan1 & ZHANG YuKui2 1Department of Chemistry , Institutes of Biomedical Sciences,Fudan University,Shanghai 200433,China,2Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023 |
| Abstract: | This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chroma-tographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hour... |
| Keywords: | proteolysis enzymatic digestion chromatographic separation peptide enrichment mass spectrometry |
| 本文献已被 CNKI 等数据库收录！ | |